Career Occupation

Scientist , Molecular Biology

Pharma and Biotech

Performing cell culture activities and laboratory techniques including aseptic expansion of mammalian cell lines , handling primary cells and immortal cells. Routine transfections , high throughput transfections and mycoplasma testing Initiate , plan and execute scientific research with emphasis on protein or DNA delivery methods (transformation , electroporation , transfection or transduction). High - throughput molecular cloning using recombinant DNA technologies Molecular cloning (include ligation independent methods) , plasmid vector construction , DNA / RNA modification enzyme use , mutagenesis , selection systems) , genome editing. Independently complete experiments using these methodologies - PCR Purification , Restriction , Ligation , Transformation , Miniprep , Colony PCR , Sequencing Keeping thorough record of cell culture parameters and results into data sheets and / or electronic note books. Responsible for data integrity and accuracy. Other functions such as writing technical protocols and filling out order forms. Deliver high quality results according to project schedule in support of product commercialization during early feasibility , development and validation phases. Should have a clear understanding of recombinant DNA technologies Should have a clear understanding of different molecular cloning and gene engineering techniques Knows therory , has full command of gene engineering methods (cloning methods (include ligation independent) , plasmid vector construction , DNA / RNA modification enzyme use , mutagenesis , selection systems) , genome editing. Strong understanding and hands - on experience of cell - culture and DNA delivery methods Should have a clear understanding and laboratory skills in the area of immunoassays like western blot , ELISA , and immunofluorescence GLP experience , documentation and automation Publications in peer - reviewed journals would be a bonus. Good understanding of research projects using standard techniques and protocols. Should be a good team player , ready to learn new techniques and innovative thinker. Industry experience , bioinformatics and grant writing skills will be considered as a bonus.

Medium Demand

Recommended Skills (6)

Scientist II Automation Analytical GLP Molecular biology Life sciences

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